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STAT proteins Signal transducer and activator of transcription belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor Activation of STAT4 induces dimerization nuclear
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Rabbit polyclonal to STAT4 Phospho Tyr693 The protein encoded by this gene is a member of the STAT family of transcription factors In response to cytokines and growth factors STAT family members are phosphorylated by
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The Alpha SureFire Ultra Multiplex kits allow the rapid sensitive and quantitative detection of two phosphoprotein targets in each well of an assay plate This kit can be used in combination with any AlphaLISA SureFire
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STAT4 phospho Tyr693 Antibody raised in Rabbit validated in WB IHC in Human Mouse Rat
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Phospho STAT4 Tyr693 Polyclonal Antibody for Western Blot IF ICC ELISA
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The STAT4 (Phospho-Tyr693) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor STAT4 (Phospho-Tyr693) protein expression profile in cells. The kit can be used for measuring the relative
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The protein encoded by this gene is a member of the STAT family of transcription factors In response to cytokines and growth factors STAT family members are phosphorylated by the receptor associated kinases and then
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Rabbit polyclonal to Stat4 phospho Tyr693 antibody conjugated to FITC Isotype Note IgG Host Note Rabbit Conjugation Note FITC Reactivity Note Human Mouse Rat Application Note IF ICC
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Image Search Results
Journal: Cell reports
Article Title: ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis
doi: 10.1016/j.celrep.2021.109602
Figure Lengend Snippet: (A and B) Heatmap for JAK family gene expression in Th1 and Th17 conditions from siCTL and siZEB1 cohort (human, (A) or WT and CD4 Cre ZEB1 L/L mice (B). (C) Western blot showing the expression of JAK family protein in human CD4 + T cells in Th17 conditions from siCTL and siZEB1 cohort at 12, 24, 48, and 72 h after nucleofection. (D) Western blot showing JAK2 expression in Th17 cells from WT and CD4 Cre ZEB1 L/L mice. (E and F) Western blot showing JAK2, total STAT4, and phosphorylated-STAT4 [pSTAT4 (pTyr693)] expression in Th1 cells from human siCTL and siZEB1 cohorts (E) or WT and CD4 Cre ZEB1 L/L mice (F). (G) Flow cytometry showing pSTAT4 expression in Th1 cells from WT and CD4 Cre ZEB1 L/L mice. (H and I) GSEA plot demonstrating the effect of ZEB1 loss on the expression of an IL12-STAT4 gene signature in (H) human and (I) mouse CD4 + Th1 cells. NES, normalized enrichment score. (J and K) Transcription level of TBX21 and IFNG in (J) human siCTL- and siZEB1-treated cells or (K) mouse WT and CD4 Cre ZEB1 L/L cells in Th1 conditions. (L and M) Flow cytometry evaluating JAK2 re-expression on Th17 differentiation in siZEB1 nucleofected CD4 + naive T cells. The contour plots (gated by GFP + ) are representative 3 different human donors, summarized in (M). Representative results from at least two individual experiments are shown in (C)–(F). Statistical differences in (G) were determined using unpaired Student’s t test (two-tailed). Statistical differences in (M) were tested using paired Student’s t test (two-tailed). NS, not significant; *p < 0.05. p values in (H) and (I) were calculated using Wald test and adjusted using the Benjamini-Hochberg method.
Article Snippet: Antibodies were from
Techniques: Expressing, Western Blot, Flow Cytometry, Two Tailed Test